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KMID : 0359319990390020338
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Korean Journal of Veterinary Research
1999 Volume.39 No. 2 p.338 ~ p.342
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Epidemiological analysis of Escherichia coli O157 : H7 by pulsed - field gel electrophoresis and multiplex polymerase chain reaction
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Jung, Byeong Yeal
Jung, Suk Chan/Cho, Dong Hee/Kim, Jong Yeom/Kim, Bong Hwan
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Abstract
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Twenty three strains of Escherichia (E) coli O157 ; H7 isolated from Korea, Japan, USA were analyzed by pulsed-field gel electrophoresis (PFGE) of XbaI-digested chromosomal DNA and multiplex polymerase chain reaction. Various PFGE patterns of E. coli O157 : H7 were found on the same farm. Most of the E, coli O157 : H7 strains had shiga-like toxin (slt) II gene only (43.5%) or both slt I and slt II genes (30.4%). eaeA gene was highly conserved in the E. coli O157 : H7. There was no correlation between PFGE and slt gene patterns. The results indicate that various genotypes of E. coli O157 : H7 have spread throughout the country and genomic DNA patterns generated by PFGE are highly specific for different strains and have significant value in epidemiologic investigations of infectious disease outbreaks.
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